Modeling of Amino Acid Separation Process in Single and Dual Chiral Ligand Exchange Chromatographic Systems

In the present paper the separation of three amino acids applying ligand exchange chromatography in single and dual chiral systems was modeled using the dispersive equilibrium model. The investigated systems consist of a commercially available non chiral column and a commercially available chiral column using a chiral mobile phase. With the experimentally determined model parameters and the multi-component competitive Langmuir adsorption isotherm a very good mathematical prediction of the break-through curves and analytical injections at low concentrations was achieved. At higher concentrations the model application is limited due to displacement effects and system complexity.